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Recognition and inhibition by humoral innate immunity pattern recognition molecules
The humoral arm of innate immunity includes diverse molecules with <em>antibody</em>-like functions, some of which serve as disease severity biomarkers in coronavirus disease . The present study was designed to conduct a systematic investigation of the interaction of human humoral fluid-phase pattern recognition molecules (PRMs) with severe acute respiratory syndrome coronavirus. Of 1 2 PRMs tested, the long pentraxin 3 (PTX3) and mannose-binding lectin (MBL) bound the viral nucleocapsid and…
The Perspective of DMPK on Recombinant Adeno-Associated Virus-Based Gene Therapy: Past Learning, Current Support, and Future Contribution
Given the recent success of gene therapy modalities and the growing number of cell and gene-based therapies in clinical development across many different therapeutic areas, it is evident that this evolving field holds great promise for the unmet medical needs of patients. The recent approvals of Luxturna® and Zolgensma® prove that recombinant adeno-associated virus (rAAV)-based gene therapy is a transformative modality that enables curative treatment for genetic disorders. Over the last decade,…
101 steps to better histology
From the patient to the pathologist, the preparation of tissue samples for histological examination requires special care, skill and proper procedures. This guide provides practical advice on sample processing techniques and summarizes best practices and suggests simple ways to avoid common mistakes. Each aspect of the histological process is described: sampling, slicing, processing in a technon, watering, slicing and staining (routine, special, immunohistochemistry and in situ hybridization). We believe that each…
Saliva or Blood for Genomic Studies?
Increasingly, saliva is emerging as an alternative to blood for DNA sampling for genomic studies. The comfort in the collection of the sample in a non-invasive way, is a point in its favor facing the patient. On the other hand, its stability at room temperature facilitates both the storage and the transport of the samples against the complex logistics that blood samples require due to the need to maintain the…
Anticancer Effect of Radix Astragali on Cholangiocarcinoma In Vitro and Its Mechanism via Network Pharmacology.
BACKGROUND This study used network pharmacology method and cell model to assess the effects of Radix Astragali (RA) on cholangiocarcinoma (CCA) and to predict core targets and molecular mechanisms. MATERIAL AND METHODS We performed an in vitro study to assess the effect of RA on CCA using CCK8 assay, the Live-Cell Analysis System, and trypan blue staining. The components and targets of RA were analyzed using the Traditional Chinese Medicine Systems…
Engaging with Lyell: Alfred Russel Wallace’s Sarawak Law and Ternate papers as reactions to Charles Lyell’s Principles of Geology.
Alfred Russel Wallace (1823-1913) and Charles Darwin (1809-1882) are honored as the founders of modern evolutionary biology. Accordingly, much attention has focused on their relationship, from their independent development of the principle of natural selection to the receipt by Darwin of Wallace’s essay from Ternate in the spring of 1858, and the subsequent reading of the Wallace and Darwin papers at the Linnean Society on 1 July 1858. In the events of 1858 Wallace and…
Herpes Simplex Type 2 (HSV-2)
HSV-2 Information HSV-2 prevalence isAlso, Generally Speaking, the highest at Africa and the Americas, Reduced in western and southern Europe than in northern Europe and North America, and lowest in Asia. HSV-2 and -1 incidence, total and by age, changes markedly by country, area within a country, and population subgroup. Age-specific HSV-2 incidence is generally higher in girls than men and in people with higher risk sexual behaviour. HSV-2 incidence…
Biology Essay
Publication involving the use of enzyme-linked immunosorbent assays or Elisa. 96 Well Elisa test kits for Scientific Life Science Research ImunoAssays Research results involving EIA enzyme immunoassay oe Eia Clia or Chemiluminescent Immuno Assay Maglumi Gentaur automation of light emission on serum in routine labs 185 parameters available COV2 IgM/IgG more parameters than Roche/Cobas, Diasorin/Liaison or Abott/Architect or Siemens/Centaur What’s Wrong with Evolutionary Causation? This review essay reflects on recent…