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Recognition and inhibition by humoral innate immunity pattern recognition molecules
The humoral arm of innate immunity includes diverse molecules with <em>antibody</em>-like functions, some of which serve as disease severity biomarkers in coronavirus disease . The present study was designed to conduct a systematic investigation of the interaction of human humoral fluid-phase pattern recognition molecules (PRMs) with severe acute respiratory syndrome coronavirus. Of 1 2 PRMs tested, the long pentraxin 3 (PTX3) and mannose-binding lectin (MBL) bound the viral nucleocapsid and…
The Perspective of DMPK on Recombinant Adeno-Associated Virus-Based Gene Therapy: Past Learning, Current Support, and Future Contribution
Given the recent success of gene therapy modalities and the growing number of cell and gene-based therapies in clinical development across many different therapeutic areas, it is evident that this evolving field holds great promise for the unmet medical needs of patients. The recent approvals of Luxturna® and Zolgensma® prove that recombinant adeno-associated virus (rAAV)-based gene therapy is a transformative modality that enables curative treatment for genetic disorders. Over the last decade,…
101 steps to better histology
From the patient to the pathologist, the preparation of tissue samples for histological examination requires special care, skill and proper procedures. This guide provides practical advice on sample processing techniques and summarizes best practices and suggests simple ways to avoid common mistakes. Each aspect of the histological process is described: sampling, slicing, processing in a technon, watering, slicing and staining (routine, special, immunohistochemistry and in situ hybridization). We believe that each…
Saliva or Blood for Genomic Studies?
Increasingly, saliva is emerging as an alternative to blood for DNA sampling for genomic studies. The comfort in the collection of the sample in a non-invasive way, is a point in its favor facing the patient. On the other hand, its stability at room temperature facilitates both the storage and the transport of the samples against the complex logistics that blood samples require due to the need to maintain the…